Eastern Equine Encephalitis (EEE) has been confirmed in Middlesex County, Ontario. On September 21, 2024, a yearling Draft filly developed acute neurologic signs (obtunded, ataxic). She died later that day. EEE was confirmed on October 7, 2024. The horse was not vaccinated against EEE. This is the 21st case of equine EEE in Ontario this year.